Robert Bruce Merrifield

Robert Bruce Merrifield (July 15, 1921 – May 14, 2006) was an American biochemist who won the Nobel Prize in Chemistry in 1984 for the invention of solid phase peptide synthesis.[1]

Early life

He was born in Fort Worth, Texas, on 15 July, 1921, the only son of George E. Merrifield and Lorene née Lucas. In 1923 the family moved to California where he attended nine grade schools and two high schools before graduating from Montebello High School in 1939. It was there that he developed an interest both in chemistry and in astronomy.

After two years at Pasadena Junior College he transferred to the University of California at Los Angeles (UCLA). After graduation in chemistry he worked for a year at the Philip R. Park Research Foundation taking care of an animal colony and assisting with growth experiments on synthetic amino acid diets. One of these was the experiment by Geiger that first demonstrated that the essential amino acids must be present simultaneously for growth to occur.

He returned to graduate school at the UCLA chemistry department with professor of biochemistry M.S. Dunn to develop microbiological methods for the quantitation of the pyrimidines. The day after graduating on 19 June, 1949, he married Elizabeth Furlong and the next day left for New York City and the Rockefeller Institute for Medical Research.

Career

At the Institute, later Rockefeller University, he worked as an Assistant for Dr. D.W. Woolley on a dinucleotide growth factor he discovered in graduate school and on peptide growth factors that Woolley had discovered earlier. These studies led to the need for peptide synthesis and, eventually, to the idea for solid phase peptide synthesis in 1959. In 1963, he was sole author of a classic paper in the Journal of the American Chemical Society in which he reported a method he called solid phase peptide synthesis, which is the fifth most cited paper in the journal's history.[2]

In the mid-60s Dr. Merrifield's laboratory first synthesized bradykinin, angiotensin, desamino-oxytocin and insulin. In 1969, he and his colleague Bernd Gutte announced the first synthesis of the enzyme, ribonuclease A. This work proved the chemical nature of enzymes.

Dr. Merrifield's method greatly stimulated progress in biochemistry, pharmacology and medicine, making possible the systematic exploration of the structural bases of the activities of enzymes, hormones and antibodies. The development and applications of the technique continued to occupy his laboratory, where he remained active at the bench until recently. In 1993, he published his autobiography, "Life during a Golden Age of Peptide Chemistry." He received the Association of Biomolecular Resource Facilities Award for outstanding contributions to Biomolecular Technologies in 1998.

Personal life

After raising their six children, James, Nancy, Betsy, Cathy, Laurie and Sally, his wife Elizabeth (Libby), a biologist by training, joined the Merrifield laboratory at Rockefeller University where she worked for over 23 years.

After a long illness R. Bruce Merrifield died on May 14, 2006 at the age of 84 in his home in Cresskill, New Jersey.[3] He is survived by his wife, children and 16 grandchildren.

References

1. ^ Kenneth Chang. "R. Bruce Merrifield, Who Won Nobel Prize in Chemistry, Dies at 84", The New York Times, 2006-05-06. Retrieved on 2008-04-12.

2. ^ R. B. Merrifield (1963). "Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide". Journal of the American Chemical Society 85: 2149. doi:10.1021/ja00897a025.

3. ^ Petkewich, Rachel. "Nobel Laureate R. Bruce Merrifield Dies At 84", Chemical & Engineering News, May 23, 2006. Accessed May 9, 2007. "Robert Bruce Merrifield, a biochemist who won the 1984 Nobel Prize in Chemistry for a method he named solid-phase peptide synthesis, died on May 14 at his home in Cresskill, N.J., after a long illness. He was 84."